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VOLUME 10 , ISSUE 3 ( September-December, 2023 ) > List of Articles

Original Article

Possible Synergism between Ampicillin and Some Pain-reducing Drugs against Escherichia coli

Thoraa Abdul Amir Drees, Falah Hasan Obayes AL-Khikani, Haider Hussein Yas Khudair, Haider Abdul Hussein Nuri, Ali Amer Hussein

Keywords : Ampicillin, Escherichia coli, Dexamethasone, Diclofenac sodium, Flagyl, Paracetamol, Synergism

Citation Information : Drees TA, AL-Khikani FH, Khudair HH, Nuri HA, Hussein AA. Possible Synergism between Ampicillin and Some Pain-reducing Drugs against Escherichia coli. Bengal Physician Journal 2023; 10 (3):79-82.

DOI: 10.5005/jp-journals-10070-8020

License: CC BY-NC 4.0

Published Online: 21-12-2023

Copyright Statement:  Copyright © 2023; The Author(s).


Background: Escherichia coli (E. coli) is one of the most common human bacterial pathogens implicated in antibiotic resistance, especially in the last decades. The antimicrobial combination is important to reduce these resistances. So the aim of this study is finding a possible synergism between ampicillin and other drugs to treat E. coli infections. Materials and methods: The present study was conducted for the period from January 2023 to March 2023 on bacteria E. coli. The tested bacteria isolated and identified by using standard bacteriological methods. Detection antibiotic sensitivity pattern of isolates was determined by agar well diffusion method. The antibiotics (flagyl, paracetamol, dexamethasone, diclofenac sodium, and ampicillin) were added separately in one dish, and then added in another dish combined with the ampicillin to know the effect of antibiotics on the growth of bacteria before and after the addition of ampicillin. Results: Twenty E. coli were isolated from various body sites. The study showed that females were more infected (70%) with E. coli than males (30%). Flagyl, paracetamol, and dexamethasone were resistant to all isolates (100%). But diclofenac sodium was resistant to 85% isolates. Resistance to ampicillin was 50%. Ampicillin showed synergy after combination with other agents but still nonsignificant (p > 0.05). The addition of flagyl increased the effect of ampicillin to 15.35 mm while it was 13.75 mm before the combination (p = 0.53). The addition of paracetamol increased the effect of ampicillin to 15 mm while it was 13.75 mm before the combination (p = 0.63). The addition of dexamethasone increased the effect of ampicillin to 15.55 mm, it was 13.75 mm before addition (p = 0.51). The combination of diclofenac increased the effect of ampicillin to 14.95 mm, it was 13.75 mm before addition (p = 0.64). Conclusion: Flagyl, paracetamol, and dexamethasone were resistant to all isolates (100%). But diclofenac sodium was resistant in 85% of all isolates. Ampicillin showed synergy after adding other agents but still non-significant (p > 0.05).

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