Background: Polycythemia vera (PV) is associated with the gain of a function point mutation in Janus 2 tyrosine kinase (JAK-2), leading to an increase in the activity of JAK2V617F. The anxiety associated with PV is mostly incidentally detected post diagnosis. Aim: To study the impact of JAK2V617F mutation status on the PV phenotype in terms of clinical and laboratory features. The mental anxiety of patients with PV was assessed at diagnosis and subsequently. Material and methods: A retrospective study was done on 50 patients with PV for the last 5 years. For the measurement of anxiety among patients, a self-administered questionnaire was used as a screening tool and severity measure for generalized anxiety disorder questionnaire and scores were used and graded accordingly. Results: On analysis of our 50 patients with PV. There was a significant statistical difference in terms of median age at presentation (61 vs 44.5 years; p = 0.002), median hemoglobin (Hb) at time of presentation (20.7 vs 17.5 gm/dL; p = 0.001), median total leukocyte count (TLC) at time of presentation (15,700 vs 8,100 µL; p = 0.001), median platelet counts at the time of presentation (3,20,000 vs 1,90,000 µL; p = 0.044) and low serum erythropoietin (EPO) level (p = 0.00) between JAK2 mutated and unmutated group. At one year when anxiety levels were checked in PV patients, it was found that none of the patients was severely anxious about their disease entity and few were moderately anxious. Conclusion: The age of patients and laboratory parameters such as (Hb, TLC, platelet counts, and serum EPO levels) showed a statistically significant difference between JAK2 mutated and Unmutated group. Thrombosis was also more common in JAK2V617F mutated patients. With the passage of time gradually the stress and anxiety declined among patients with PV.